I am using Thermofisher 5'-RACE PCR kit. In troubleshoot guide, the reason for incomplete sequence could be due to non-specific binding of AAP in poly-C rich region interior to end of RNA. However, there is no such region.
Hi, Can anyone tell me any tool or suggestions as i want to seperate conference name from the title of paper? I AM LOOKING FOR CONFERENCE NAME NOT JOURNAL NAME.
10 August 2020 7,934 4 View
My data set consists of 3 categorical IVs (1st IV consists of 2 categories, Second IV also consists of 2 Categories and 3rd IV consists of 3 categories).
23 July 2020 952 2 View
Hi I am developing a ranking formula for different levels of users that is beginner,Intermediate and Expert. I am using three metrics for it that is impact factor,h index and citations,We need to...
02 March 2020 4,633 4 View
It may show difference in efficiency but is it recommended or usable method for extraction of bituminous.
27 February 2020 4,446 1 View
I have sintered Mg3Zn1Ca15Nb composite at 630-degree temperature and measured the phases present in the composite through XRD. But, no phases are detected in XRD analysis. So what can be the...
23 February 2020 9,366 5 View
Emerging contaminants are present in traces in water, air and soil. these can not be removed by conventional treatment methods. ECs are classified on the basis of their causes or sources of...
31 July 2019 1,869 2 View
why is dual band antenna at 28/38 ghz more important than tow single band antennas
05 July 2019 8,666 8 View
Want to directly use non stationary time series without differencing the series.
27 March 2019 7,623 2 View
Gel extraction from aloe Vera is performed generally centrifugal temperature at various speed. what are the effects of temperature variation on the extraction and properties of gel???
26 January 2019 419 6 View
there carbons are used for the production of super capacitor. If anyone know about this, please share information or papers.
26 January 2019 8,980 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View