How to run a mediation analysis with two different samples?

Hi, I tried to run a mediation analysis in which the DV is made up of test scores after a training in the experimental group VS the control group, and in which the mediator is measured by a...

17 November 2020 7,178 3 View

How can I add a chain identifier to a PDB file generated by Phyre2?

I am trying to use DynaMut2 to study stabilizing/destabilizing effects of mutations in a protein. I generated a 3D structure using Phyre2, and it gave me a PDB file without a chain identifier....

22 September 2020 495 11 View

Survey data collection sample purchase - Qualtrics or Dynata?

I am planning for a survey research project (national representative sample for 15 min survey) and am considering which panel/sampling company to use. I have used Dynata before, they are...

22 September 2020 9,334 4 View

Protocol for using Attune NxT Acoustic Focusing Cytometer to detect Ploidy level in leaf tissue?

Hello, I am looking for a detailed protocol to use the Attune NxT Acoustic Flow cytometer to determine ploidy level in plant samples with PI staining. Details for laser, threshold, and...

21 September 2020 8,334 2 View

Can a study have multiple research philosophies ?

I would like to know whether a study can have multiple research philosophy. I am currently studying the influence of ownership on firm performance through regression analysis. I am using case...

19 April 2020 1,073 3 View

How do you define the ontology and epistemology of mixed methods research?

The research which i am doing consists of multiple regression and case studies research. I would like to know whether what i am doing is a mixed methods. Also, what are its ontological and...

08 April 2020 5,457 15 View

Somebody know a fast DNA extraction method for plant for to do in the field?

I am looking found reference about people that has been working the proposed method in this publication: "Nucleic acid purification from plants, animals and microbes in under 30 seconds" from Zou...

10 March 2020 860 2 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

How do I isolate LC nucleus for cell culture?

I need to isolate LC for primary cell culture? How should I do? What is better: use frozen tissue or fresh tissue?

21 January 2019 9,383 3 View

What happen if I put enzyme on optimum pH and temperature but I did not put any substrate to digest / hydrolyse?

I'm doing BSA test and I need a blank to identify the product of my substrate (which means I can't use water since BSA will read my enzyme as protein as well) ; problem is, once I substract the...

07 November 2018 5,006 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View