Langerhans Dendritic cells are APC in the skin. They have a Haemostatic role in the Immune system.In spite of many works on these type of cells, Nobody knows their exact function in immuno-tolerance.
It´s very interesting. Would yo be disposed to send me results at the end of your work?.
Best regards.
Dr Bavio Enrique.-
If you are working on a mouse model, I suggest you isolate dendritic/Langerhans cells from the mouse ear. There are many protocols in the literature.
Actually, the right answer to your questions relais on many factors mostly related to the purpose of your research. Langerhans cells are particularly important in the context of skin immunity to different pathogens and contact agents. These cells exhibit a particular flexibility of responses. Isolation from intact skin using collagenase would provide you fresh LC. Anyway, you probably would need to compare the response of LC with that of other DC cells.
I agree with Mario and Fredy, the study of Langerhans cells must be done from explants: the inner ear of the mouse and the human skin resulting from cosmetic surgeries are the best sources of dendritic cells of Langerhans .
However, the number of cells obtained is rather low and requires rather complex purification steps.
In vitro cells can also be generated from CD34 + progenitors in humans and bone marrow cells in mice. The cytokine cocktail required to generate langerhan cells is GM-CSF + TNFα, to which TGFb is added to stabilize the Birbeck granules.
The Birbeck granules that are involved in the capture of pathogens (bacteria, HIV, CMV ...) are visualized with CD207 antibodies that target Langerine, the main constituent of the granule. The functional langerhans cells are therefore qualified with a double Class II / CD207 marking.
Finally for most functional tests the CD207 antibodies will be used as positive controls for activation, inhibition or facilitation: for example for HIV and Lcells studies, DCGM4 / 122D5 antibodies (activation), 808E10 (inhibition) and 109A2 (facilitation).
But there are many other ways of signaling
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