I need to know the reason for serum starvation of cell line before performing the in vitro assay for LPS stimulation and then treatment with CB2 receptor agonist
Hello Priyansha Singh
Serum starvation is common in cell-based assays. As mentioned by Shima Lotfollahzadeh
, serum starvation will help to synchronize the cells to the same cell cycle phase. So, when you add the agents to the cells, theoretically you have removed the impact that the cell cycle would have on the cells’ response to the treatment.Serum contains a number of substances which include proteins, cytokines, growth factors and other small molecules. You need to serum starve the cells in such a way that it does not affect the health status of your cells. The duration of serum starvation will vary with cell type and can include anything from 15 - 30 mins up to 48 hours. So, you need to optimize the period of serum starvation for your cells before you perform your experiment.
I would suggest that serum starvation be carried out in a medium with low serum concentration (relative to normal growth medium), typically 0.1–0.5%, but sometimes as high as 1-2% or as low as 0.05%. Low serum concentration in the culture medium will help cells to remain healthy but not proliferate.
Best.
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