Hello Priyansha Singh

Serum starvation is common in cell-based assays. As mentioned by Shima Lotfollahzadeh

Serum contains a number of substances which include proteins, cytokines, growth factors and other small molecules. You need to serum starve the cells in such a way that it does not affect the health status of your cells. The duration of serum starvation will vary with cell type and can include anything from 15 - 30 mins up to 48 hours. So, you need to optimize the period of serum starvation for your cells before you perform your experiment.

I would suggest that serum starvation be carried out in a medium with low serum concentration (relative to normal growth medium), typically 0.1–0.5%, but sometimes as high as 1-2% or as low as 0.05%. Low serum concentration in the culture medium will help cells to remain healthy but not proliferate.

Best.