16 Questions 4 Answers 0 Followers
Questions related from Priyansha Singh
Please tell such properties in terms of the genetic expression of those markers of HepG2 cell lines which makes them a suitable in-vitro model for mitochondrial toxicity & cytotoxicity studies
02 December 2023 4,773 1 View
We are standardizing a protocol for studying cytotoxic drugs (mainly anticancer class of drugs) and we are using HepG2 cell line for that. My question is why only HepG2 cells while we can use...
02 December 2023 6,376 3 View
Today I saw my T25 flask and I saw my HepG2 cells to be clumped and forming layers and I could not guess what went wrong during trypsinization
17 November 2023 7,802 2 View
please refer to me the protocol of insertion of PXR gene in existing DNA which has to be transfected in HEK293 WT cell line
28 October 2023 7,893 4 View
I am treating HEK293 WT with a compound and I am using phenol red-free DMEM for this but for the maintenance of T25 flask of the same treated cells I use complete DMEM
28 October 2023 4,414 0 View
I am working on TRPML receptors. I need to know the answer of this
30 September 2023 756 1 View
I have come across the principle of pH meter but why do we select 3 molar only ?
24 July 2023 5,122 0 View
in preclinical studies on rats for the study of beta caryophyllene olive oil is mostly used as a vehicle is it due to the pharmacokinetic parameters ?
27 May 2023 875 0 View
I am studying the hepatoprotective activities of beta caryophyllene therefore i need to know about the route of clearance of beta caryophyllene
30 April 2023 7,886 1 View
in the given protocol it says that we need to add 750 microliter thiobarbituric acid (TBA), 750 microliter acetic acid (20%), 100 microliter of sample and 100 microliter of SDS
28 April 2023 7,264 0 View
I am preparing myself for western blot of my samples, but I have confusion in preparing the protein samples because there are a lot of protocols given and I am using RIPA buffer specifically for...
26 April 2023 6,011 2 View
I am doing RNA extraction for further RT- PCR
05 April 2023 6,937 3 View
i need to perform western blot and i was looking out for some antibodies separately for activated hepatic stellate cells, hepatic Kupffer cells, sinusoidal endothelial cells, and hepatic fibrosis.
22 January 2023 6,437 0 View
I need to know the reason for serum starvation of cell line before performing the in vitro assay for LPS stimulation and then treatment with CB2 receptor agonist
17 January 2023 8,567 1 View
I am doing research on hepatoprotective properties of beta caryophyllene but i wish to know the volume of distribution and the clearance rate of beta caryophyllene
13 December 2022 430 0 View
How we select the animal for particular preclinical study
29 September 2022 2,349 5 View
