Priyansha Singh

6 Questions 2 Answers 0 Followers

Questions related from Priyansha Singh

What is the renal and hepatic clearance of beta caryophyllene ?

I am studying the hepatoprotective activities of beta caryophyllene therefore i need to know about the route of clearance of beta caryophyllene

30 April 2023 7,822 1 View

What is the general protocol for sample preparation in western blotting ?

I am preparing myself for western blot of my samples, but I have confusion in preparing the protein samples because there are a lot of protocols given and I am using RIPA buffer specifically for...

26 April 2023 6,001 2 View

In RNA extraction why do we add cold isopropanol ?

I am doing RNA extraction for further RT- PCR

05 April 2023 6,933 3 View

What markers of activated hepatic stellate cells, hepatic Kupffer cells, sinusoidal endothelial cells should i use for western blotting ?

i need to perform western blot and i was looking out for some antibodies separately for activated hepatic stellate cells, hepatic Kupffer cells, sinusoidal endothelial cells, and hepatic fibrosis.

22 January 2023 6,424 0 View

I am performing the invitro analysis of CB2 receptor agonist on RAW264.7 macrophage cell line. Why does the protocol says to serum starve the cells?

I need to know the reason for serum starvation of cell line before performing the in vitro assay for LPS stimulation and then treatment with CB2 receptor agonist

17 January 2023 8,560 1 View

On what basis we use animals in pre clinical studies?

How we select the animal for particular preclinical study

29 September 2022 2,338 5 View