To do serial dilution, is it necessary to have stock solution and diluted solution with same solvent? Or, they can be different?

I have a powdered compound which is not readily soluble in serum containing medium. To dissolve properly, I have made a stock solution of 100 mg/ml in 1 ml PBS of that compound. If I want to make...

01 February 2020 4,368 3 View

What is the best way to dissolve a powdered natural compound to see the IC50 value?

I am going to see the IC50 value of a powdered natural supplement. To treat the cells in 96 well plate, I have to dissole them in these concentration: 0, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and...

31 December 2019 6,114 3 View

My secondary antibody is placed in the "amber" vial to protect the light. While diluting this, it is exposed to light, does it matter?

My secondary antibody for western blot is placed in the "amber" vial to protect from the light. However, while making dilution of the secondary antibody it is exposed to light for 2-3 minutes....

06 July 2019 349 4 View

I am searching for a FDA approved drug which targets the Galectin-3 protein in metastatic breast cancer. Is there any website or fact sheet available?

I am searching for a FDA approved drug which targets the Galectin-3 protein in metastatic breast cancer. Is there any website or fact sheet available to find out exactly that information?

06 July 2019 4,849 2 View

Why does my blot give this kind of hazy look?

I use stainfree gels. I have attached here one of my good blots and hazy blots. Can anyone give me any idea why this hazy look appears instead of clear one?

04 May 2019 4,104 4 View

While loading the western blot samples in the gels, is it necessary to keep the samples on ice? Or thawing in room temperature is okay?

Our practice is thawing the western samples from -80 to room temperature or thawing in the 4 degree for a while. What is the right practice of keeping the western samples during loading in the...

04 May 2019 925 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View