Md Rafi Anwar

9 Questions 19 Answers 0 Followers

Questions related from Md Rafi Anwar

Can I use 10% Bovine Calf Serum (BCS) instead of 10% Foetal Calf Serum (FBS) for MDA-MB-231?

Because of the availability and low cost of BCS, I am going to use this one for MDA-MB-231 breast cancer cell line. But, I see in most of the papers published using FBS for this cell line....

28 May 2020 2,301 0 View

To do serial dilution, is it necessary to have stock solution and diluted solution with same solvent? Or, they can be different?

I have a powdered compound which is not readily soluble in serum containing medium. To dissolve properly, I have made a stock solution of 100 mg/ml in 1 ml PBS of that compound. If I want to make...

02 February 2020 4,472 3 View

What is the best way to dissolve a powdered natural compound to see the IC50 value?

I am going to see the IC50 value of a powdered natural supplement. To treat the cells in 96 well plate, I have to dissole them in these concentration: 0, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and...

27 January 2020 406 4 View

What is the best way to dissolve a powdered natural compound to see the IC50 value?

I am going to see the IC50 value of a powdered natural supplement. To treat the cells in 96 well plate, I have to dissole them in these concentration: 0, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and...

01 January 2020 6,196 3 View

I am maintaing CL-S1 cell line. What is the exact doubling time of this cell line?

I am maintaining CL-S1 cell line. It's an adherent female epithelial mammary gland mouse cell line. It is very slow growing. Can anyone inform what is the exact doubling time for this cell line?...

08 August 2019 6,919 2 View

My secondary antibody is placed in the "amber" vial to protect the light. While diluting this, it is exposed to light, does it matter?

My secondary antibody for western blot is placed in the "amber" vial to protect from the light. However, while making dilution of the secondary antibody it is exposed to light for 2-3 minutes....

07 July 2019 429 4 View

I am searching for a FDA approved drug which targets the Galectin-3 protein in metastatic breast cancer. Is there any website or fact sheet available?

I am searching for a FDA approved drug which targets the Galectin-3 protein in metastatic breast cancer. Is there any website or fact sheet available to find out exactly that information?

07 July 2019 4,922 2 View

While loading the western blot samples in the gels, is it necessary to keep the samples on ice? Or thawing in room temperature is okay?

Our practice is thawing the western samples from -80 to room temperature or thawing in the 4 degree for a while. What is the right practice of keeping the western samples during loading in the...

05 May 2019 1,006 7 View

Why does my blot give this kind of hazy look?

I use stainfree gels. I have attached here one of my good blots and hazy blots. Can anyone give me any idea why this hazy look appears instead of clear one?

05 May 2019 4,184 4 View