I am searching for a FDA approved drug which targets the Galectin-3 protein in metastatic breast cancer. Is there any website or fact sheet available?

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Can I use 10% Bovine Calf Serum (BCS) instead of 10% Foetal Calf Serum (FBS) for MDA-MB-231?

Because of the availability and low cost of BCS, I am going to use this one for MDA-MB-231 breast cancer cell line. But, I see in most of the papers published using FBS for this cell line....

27 May 2020 2,326 0 View

To do serial dilution, is it necessary to have stock solution and diluted solution with same solvent? Or, they can be different?

I have a powdered compound which is not readily soluble in serum containing medium. To dissolve properly, I have made a stock solution of 100 mg/ml in 1 ml PBS of that compound. If I want to make...

01 February 2020 4,508 3 View

What is the best way to dissolve a powdered natural compound to see the IC50 value?

I am going to see the IC50 value of a powdered natural supplement. To treat the cells in 96 well plate, I have to dissole them in these concentration: 0, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and...

26 January 2020 434 4 View

What is the best way to dissolve a powdered natural compound to see the IC50 value?

I am going to see the IC50 value of a powdered natural supplement. To treat the cells in 96 well plate, I have to dissole them in these concentration: 0, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, 8.0 and...

31 December 2019 6,228 3 View

I am maintaing CL-S1 cell line. What is the exact doubling time of this cell line?

I am maintaining CL-S1 cell line. It's an adherent female epithelial mammary gland mouse cell line. It is very slow growing. Can anyone inform what is the exact doubling time for this cell line?...

07 August 2019 6,966 2 View

My secondary antibody is placed in the "amber" vial to protect the light. While diluting this, it is exposed to light, does it matter?

My secondary antibody for western blot is placed in the "amber" vial to protect from the light. However, while making dilution of the secondary antibody it is exposed to light for 2-3 minutes....

06 July 2019 456 4 View

While loading the western blot samples in the gels, is it necessary to keep the samples on ice? Or thawing in room temperature is okay?

Our practice is thawing the western samples from -80 to room temperature or thawing in the 4 degree for a while. What is the right practice of keeping the western samples during loading in the...

04 May 2019 1,029 7 View

Why does my blot give this kind of hazy look?

I use stainfree gels. I have attached here one of my good blots and hazy blots. Can anyone give me any idea why this hazy look appears instead of clear one?

04 May 2019 4,202 4 View

Is this website real?

Is this website real? https://isar.org.in/event/registration.php?id=2434532

08 August 2024 484 1 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license?

Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.

05 August 2024 7,098 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View