Please if someone has it, could you share it with me please?
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...
02 March 2021 4,624 4 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...
01 March 2021 3,052 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?
27 February 2021 7,063 7 View
To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...
23 February 2021 6,266 3 View
We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...
23 February 2021 1,898 1 View
As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...
23 February 2021 3,175 3 View
Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...
22 February 2021 8,211 3 View
I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...
26 February 2021 8,480 3 View
I was refining my XRD data in FullProf. Highest intensity was 400000. The fitting was good but still the Chi^2 value is very high (0.174E+04) I divided the intensity by some constant factor (say...
17 February 2021 3,383 5 View
I have a protein model and want to replace a 12-amino acids-long region within the center of the model with those of a PDB file containing a 12-amino acids long peptide of the same sequence. This...
02 February 2021 779 2 View
I am working on XRD analysis of the clay minerals from the sea sediments. My XRD pattern shows very high counts at the start of the XRD pattern (3 degree 2 theta) which is very unusual. The counts...
31 January 2021 4,493 9 View
I tried to do single-crystal Rietveld Refinement on my single-crystal XRD data using the option "refinement on single-crystal data/ integrated intensity data" in Fullprof but it is giving error...
30 January 2021 7,813 3 View
To have the desired grain size in 6061 and 6063 Al alloy, what should be the minimum % of Ti and B pick up and what is the chemistry/metallurgy behind that?
07 January 2021 9,305 2 View
Hi I wanna use Reitveld analysis in my project but i have big question that is: Should i see quality of each card (or Not important !!?), or it is enough to check the score and scale factor of...
04 January 2021 7,750 1 View
Here are three different ways to calculate crystallite size and lattice strain of a sample, based on XRD peaks: "Scherrer" calculation, "Williamson-Hall" or "Rietveld". Each calculation is proper...
04 January 2021 7,836 4 View
Hello everyone, I am aiming to write a PHD proposal of 1000 words. The research is described by the supervisor. That means, research questions, aim, and instruments are well defined by the...
01 January 2021 6,291 7 View
I am trying to refine my XRD data with Fullprof software. Although I got low values for R Bragg and Chi 2 factors, when I insert new cell parameters as phase, they don't fit all peaks. In fact,...
18 December 2020 9,322 7 View
