I am looking for a sealing materials which can be used at around 1000 celsius and 50 bar pressure. Are there any ceramic which can be gas tight?

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In SPSS, how to generate P-value of corresponding odds-ratio with 95% CI using complex sample logistic regression option?

I plan to apply multinomial logistic regression using the complex sample option of SPSS. The dependent variables have 04 categories (low, moderate, high, and very high), and 05 independent...

02 June 2024 6,967 3 View

In SPSS, how to generate P-value of corresponding odds-ratio with 95% CI using complex sample logistic regression option?

I plan to apply multinomial logistic regression using the complex sample option of SPSS. The dependent variables have 04 categories (low, moderate, high, and very high), and 05 independent...

02 June 2024 8,984 4 View

Mouse MSCs not expressing TSG-6 on cytokine stimulation?

I am using mouse bone marrow derived MSCs (passgae 3-6) for my in vitro cytokine priming experiment. My basal media is 2% FBS-DMEM. I am using 10 ng/ml TNF-a, or IFN-g or both for 24 hours. I see...

27 May 2024 8,935 0 View

Is it logically correct to calculate the Tafel slope values for Hydrogen Evolution Reaction (HER) in positive potential (vs. RHE) range?

Hi there, First of all, thanks in advance for answering my question but please ignore this if you're not a technical expertise regarding the topic. We usually draw (or, extract) the Tafel plots...

14 March 2024 975 3 View

Hi, Do I have to culture cells in all wells in 96 well plate? Could anyone please explain? How will seeds control and treated cells in 96 well plate?

How can I measure the absorbance and calculate the data of treated and non treated cells in 96 well plate? Do I have to culture cells in all 96 wells in 96 well plate? Could anyone please explain?...

18 September 2023 9,426 2 View

How neutrophil isolation on ice affect the cells and culturing subsequently?

I am using a magnetic separation based isolation kit for mouse neutrophils and the protocol mentions 2 incubation on ice for 15 min each. I am aware that low temperature pre-activate the...

05 September 2023 6,293 0 View

How do you differentiate a synteny and a multiple sequence alignment?

In terms of genome and genetic arrangements

31 August 2023 3,567 2 View

In gaussian, why are the numbering of normal modes opposite in case of anharmonic frequency calculation?

I am running anharmonic frequency calculation in gaussian 16. In the output file, I got the frequencies along with their normal modes in a section named Vibrational Energies at Anharmonic Level....

28 June 2023 7,564 0 View

How to prepare monkey tissue for western blot for seeing the Mu opioids receptor protein?

Hi Everyone, I am trying to do the Western Blot for looking over Mu opioid receptor protein. I am unable to find the suitable article/protocol to do that. I am wondering is there anyone who can...

10 October 2022 5,575 0 View

How to get ready for a Master's Thesis?

Hello All, This is my pleasure to share that I am going to start a Thesis for my MSc in Climate Change and Development in Spring 2022. The groundwork has already been initiated, got the...

04 January 2022 7,493 5 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View