I am having problems transferring my 420 bp long gene to ppizα by applying heat shock in its transformation.
What are your recommendations to solve this problem, apart from electroporation, and what is the measure of excess AT content in a gene?
I have carbon fiber composite specimens and wonder about the dimensions of the specimens which are 25 mm wide, 250 mm long and 10 mm thickness is compatible with the ASTM D3039 Tensile test...
08 October 2020 1,261 4 View
25 July 2016 6,507 1 View
When we use software such as autodock and chimera, the scores are around -7, -10, -12 kj. The Moldock scores are very high numerically around -100, -150. My question is; Can a conversion be made...
01 January 1970 3,285 6 View
We are running a flow cytometry center in our University Hospital, We use flow cytometry as diagnostically and for research. We think standardization has utmost importance in flow cytometric...
01 January 1970 4,454 2 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
02 March 2021 7,082 3 View
Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...
01 March 2021 6,200 3 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...
27 February 2021 1,233 1 View