I am having a problem ligating my vector with PCR product by using the pcDNA3.1 V5-His-TOPO expression kit.Have anybody had such an experience?

07 September 2020 0 4K Report

I am trying to clone using the pcDNA3.1 V5-His-TOPO expression kit. There is no problem transferring it to the cell, but I can only transfer the vector. I did not succeed in vector-insert ligation. Have anybody had such an experience?

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