I am trying to clone using the pcDNA3.1 V5-His-TOPO expression kit. There is no problem transferring it to the cell, but I can only transfer the vector. I did not succeed in vector-insert ligation. Have anybody had such an experience?
Hello. I can´'t find a full text article that i need for my research. Can you help me? The reference of the article is: Ref ID: 3441009. Article: Adolescent Health: Communication With Adolescent...
16 May 2024 3,293 3 View
Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...
12 May 2024 6,819 2 View
Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...
02 May 2024 9,588 3 View
I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...
08 April 2024 2,035 4 View
Hello! I have carried out my undergraduate research project using two pre-validated, Likert scale self report questionnaires looking at emotional intelligence and resilience. (SSEIT and...
16 March 2024 7,642 3 View
Hi, I am carrying out a very basic undergraduate research study with no stats teaching on the course (medical degree) as a result I am floundering and wonder if anyone could help. I am using two...
01 March 2024 6,741 9 View
Dear All, We used 0.45, 1.2 and 2.5 uM filter papers for vacuum filtration for during the sythesis of graphene oxide with the Hummers method for washing of the material after final treatment with...
25 January 2024 3,411 5 View
I am currently working with HT-22 cells by transfecting them with bacterial plasmids to perform IHC. However, we have not had much success with reaching a 50% transfection rate. I usually do about...
17 January 2024 7,802 6 View
hi again! I had never worked with Caco-2 cells before and just recently I thaw some. When I look at them I see something that I really don't know what it is. I know these cells secrete a lot of...
14 January 2024 9,068 4 View
Hello. I plan to do a simple immunofluorescence procedure with HT-22 cells. I plan to transfect the cells and fix them. I do not believe I will have time to come in on the weekend. Is it possible...
11 December 2023 6,640 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View