How to convince the public to wear masks/gloves if they are resistant?

As you all know, this pandemic has taken a major toll on the entire global community. In some societies, people do abide by COVID protective measures. Yet, in other societies people are resistant...

30 September 2020 546 13 View

What are these additional bands in my agarose gel results?

I have included pictures to illustrate my point. In the gel image that has only 4 lanes: Lane 1 is a 1 kb molecular weight ladder. Lane 3 is my wild type DNA (in a pGEX plasmid) and Lane 4 is the...

28 August 2020 4,146 4 View

Delta Furin SARS-COV-2 mutant Western Blot Analysis showed double band around 250 kDa. Processed and unprocessed version? Need evidence, please help?

I analysed SARS-CoV-2 Spike Protein delta Furin mutant in my bachelors thesis and need evidence (paper, research or something like that) to proof my suggestion. I think the double band at around...

26 August 2020 1,058 4 View

How to get SD from CI??

I apologize in advance for my rookie question. I have been looking in the internet how to get SD from CI values, and some sources tell me to apply the formula =SQRT(N)*(CI_upper.limit -...

19 August 2020 8,946 2 View

Who I should contact to get permission to use the BREQ-2 tool in academic research?

The BREQ-2 tool is commonly used in physical activity research to measure motivation. The tool was developed by David A. Markland. I have not been able to find how to ask consent for its use in...

15 April 2020 5,428 3 View

How to aggregate data from various surveys and reflect them onto one person?

So for example, we have the following polls: First poll: People who said they like orange juice for breakfast are as follows: Texas (50%), NY (25%), North Carolina (20%), and Florida (5%) Second...

01 March 2020 2,916 3 View

How do voter data companies collect or match info?

I have been recently studying proprietary voter files and data. While I know that voter files are for the most part public (paid or free), I am confused as to how companies match this data to...

30 January 2020 7,520 1 View

Is Bowtie2 assembly file saving problem caused by no space left on the disk?

Hello, Here is what I got with Bowtie2 while changing .sam file to .bam file: "samtools view -bS egpart1.sam > egpart1.bam [E::bgzf_flush] File write failed (wrong size) samtools view: writing to...

28 December 2019 3,244 2 View

What type of design?

I am asking for a student I'm mentoring. It's an action research project with a small sample of maybe 8-10 participants. If the student includes a likert attitude scale but is mostly focusing on...

08 June 2019 9,553 4 View

I am looking for research topic ideas for a systematic review related to electronic medical records?

There are systematic reviews that have explored the barriers and facilitators that influence the success of EMR adoption. I am looking for other research topic ideas for a systematic review...

22 May 2019 9,512 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View