Some are used by filter sterilization and some are used by autoclaving. Which is correct?
I am quite sure you can autoclave it alone and also in some mixtures at lower concentration. I would not autoclave it together with Ca2+ or Mg2+ if their concentration is critical, nort at a low pH becasue it will turn into acetic acid and partly evaporate, make sure your pH is neutral or slightly basic, which it would normally be from Na-Acetate so that's fine. It depends a bit at the final use and how much do you need, if you need liters it is a pain to squeeze through a filter but just a few cl is ok, make sure you filter into a sterile receptacle.....
@Emma, I also did that, usually at pH 5-5.4 and that was fine for the DNA work, but that was also pure I think.
@puhazh, How do you want to use it in the end, big cultures, liquid/solid medium? Please give us feedback on what you choose and if it worked.
am going to add 5 ml to 95 ml media for my interest of concentration. the 95 ml of media consist CaCl2 and Mg2So4. i think it is better to autoclave sodium acetate and medium separately
Yep if your medium contains Ca and Mg I would autoclave seperately. thanks for the feedback and goodluck
Depends on what you prefer but sodium acetate is very much autoclaveable
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology