You can try this

Wash Buffer

1 mM EGTA, pH 8.0

Filter through a 0.22 μm filter before use.

Add the DTT, PMSF, and Na2S2O5 fresh before use.

200 mM KCl

50 mM Tris-HCl, pH 8.0

1 mM EDTA, pH 8.0

2 mM Sodium Metabisulfite (Na2S2O5)

0.5 mM DTT

1 mM PMSF

10% (v/v) Glycerol

Elution Buffer

1 mM EGTA, pH 8.0

Filter through a 0.22 μm filter before use.

Add the DTT, PMSF, and Na2S2O5 fresh before use.

200 mM KCl

50 mM Tris-HCl, pH 8.0

1 mM EDTA, pH 8.0

2 mM Na2S2O5

10 mM Maltose

0.5 mM DTT

1 mM PMSF

10% (v/v) Glycerol

Thank you Nilanjan Roy. I will try these buffers. But my protein requires a cation metal to be in functional form. Can I still use EDTA?