Sasikala Muthusamy

4 Questions 7 Answers 0 Followers

Questions related from Sasikala Muthusamy

Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

07 April 2020 4,450 4 View

How to avoid lumen (or whole) formation while culturing intestinal epithelial cells (N87)?

I culture N87 cells, suddenly their morphology has changed from smooth monolayer to many lumen containing colonies even at 20 - 30% confluency. Any suggestions is highly appreciated. Thanks in...

11 October 2018 7,551 0 View

What is the best method to process transwell (PET 0.4um inserts, corning) grown polarized monolayer cells for immunofluorescence studies?

I am establishing a polarized gastric-epithelial monolayer culture on transwell system for bacterial infection studies. I use NCI-N87 cells and I culture them by replacing medium on every...

23 February 2018 3,598 9 View

I am facing a problem in binding and elution of an MBP tagged recombinant protein from MBP column. Can anyone lend their expertise?

I use 5X 0.5M HEPES buffer and 100mM ZnSo4 Nacl buffer into 1x as column buffer with pH 7.4 and 1X column buffer with 0.5M maltose as an elution buffer. Please suggest how I can overcome this...

16 April 2014 8,375 2 View