Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

What are effects of steel jacketing on flexural strength?

Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...

22 February 2021 1,906 5 View

How to model steel jackets using SeismoStruct?

Hi all, Does SeismoStruct support modeling rectangular RC columns retrofitted with steel jackets (e.g. rectangular, elliptical) ? Any suggestions for other software (apart from...

20 February 2021 4,521 1 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

What Buffer should i use for T4 virus for electron microscopy ?

Hello, I currently have my T4 virus in HEPES buffer. Unfortunately, there are always crystals that are contaminating my solution (due to the buffer in which the Viruses are) when I observe them...

01 February 2021 400 3 View

Can we use control variables during Structural Equation Modeling with Amos?

We want to explore a mediating relationship employing SEM with Amos SPSS. Our independent variable is attitude toward wife abuse. mediator is exposure to male marital violence, and outcome...

07 January 2021 4,481 3 View

Response Surface Methodology?

I'm using 3 different light intensities (constant values 15,30,45 W) for the wastewater treatment of an organic compound and optimizing the parameters (pH, catalyst dose and light intensity)...

31 December 2020 2,377 4 View

How to simulate a varying load /load profile in Simulink/MATLAB?

I am looking for a block in MATLAB that can simulate a varying load such as a load of a house given below. this block will be connected to a power system

27 December 2020 5,835 5 View

Is Psychotherapy effective for children than adult? Why? Is there any good article to respond that?

I study and research about anxiety disorders in children in Iran and I sometimes face questions like this and unfortunately I could not find article or book to answer that.

30 October 2020 6,822 7 View

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30 October 2020 961 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View