Nelofar Karim

5 Questions 16 Answers 0 Followers

Questions related from Nelofar Karim

How to fix Embryoid bodies for visualization under microscope and for ICC?

This is the first time that I have made EB by hanging drop method. Its been 5 days that i have transferred them to a non tissue culture treated plate, uptill now they are not growing in size.. i...

04 October 2021 8,101 0 View

How to calculate MWCO for amicon filter for filtering virus of particular size?

I will be using amicon filter for the first time to conc. my lentiviral particles produced. The total weight of the plasmid DNA used to transfect accounts to 40.7kb. How do i calculate my produced...

16 April 2021 7,700 3 View

Debris in ipsc passaged culture plate?

I passaged my ipsc's and found that the plate had debris.. so next day i changed the media but it was still there and moreover next day the debris covered the whole area of culture plate... i...

05 December 2020 1,102 4 View

I am encountering bacterial contamination during IPSC formation when in last stage of compacting and maturing in E8 media .. Dont know why?

Inspite of taking care of almost every aspect my transduced human fibroblast cells encounter bacterial contamination problem when in E8 media + supplement ,during last stage of final IPSC...

03 December 2020 876 0 View

need to isolate plasmid DNA from transformed cells. have been using qiagen midi kit which yielded 700ng/ul but now -ve results r coming 200ng/ul ?

earlier when i used this kit some 15 days ago i got great results but now when i am trying for the same.. my results r going down. dont know the reason behind. conc. of cells.. there phase growth...

25 June 2019 4,361 4 View