I am doing inplanta BiFC to see the interaction of proteins. I am monitoring GFP expression from 2nd day onwards. I don't see GFP signa. suggestions?

10 October 2016 1 8K Report

I am Infiltering my BiFC constructs along with a localization marker. The localization marker has RFP. I can very clearly see RFP signal, but I am not able to find GFP signal so far. I have already repeated my experiments three time. Any suggestions?

Attila Lehotzky

I am not a plant experts but GFP signal is pH-sensitive. keep in your mind. i do not ask trivial questions like expression of proper constructs in tissue, etc.

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