Nisha Agrawal

2 Questions 2 Answers 0 Followers

Questions related from Nisha Agrawal

I am doing inplanta BiFC to see the interaction of proteins. I am monitoring GFP expression from 2nd day onwards. I don't see GFP signa. suggestions?

I am Infiltering my BiFC constructs along with a localization marker. The localization marker has RFP. I can very clearly see RFP signal, but I am not able to find GFP signal so far. I have...

10 October 2016 8,149 1 View

Does efficiency of gibson assembly kit decreases over time?

Hi everyone, I am doing gibson assembly and I tried 4 times but its not working. I checked my primers and all but everything looks fine. The kit which I am using is one year old. So could it be...

06 June 2016 6,270 2 View