Transwell Migration assay ?

Dear Colleagues I am bout to explore the extravasation of tumours by in vitro modelling using a trans well (Boyden chamber) assay; I must confess this is completely new to me In essence, we are...

07 May 2017 8,296 3 View

Standard curves in qPCR for very low copy number genes ?

Dear Colleague(s) I am dealing with a gene at the moment that exhibits very low expression and is barely detectable from neat cDNA. As such if you dilute the cDNA to construct a standard curve...

01 June 2016 4,189 7 View

REST Gene expression software: Windows compatibility issues ?

23 March 2016 6,328 6 View

Housekeeper choice for real time qPCR using human heart RNA ?

Dear Colleague(s) I am well versed with real time qPCR but have never actually worked with human heart tissue to determine relative expression of a GOI, viz cytochrome p450 2J2 variant. In...

14 January 2016 2,996 2 View

Does anyone know if you can perform delta delta Ct analysis on gel based bands ?

Hi, I routinely measure gene expression by qPCR using the delta delta ct method, wherein a test gene is measured in treated and untreated samples and both are normalised (for loading/amplification...

20 October 2014 5,527 7 View

Does anyone know of any research linking problem framing to communication frames?

01 January 1970 9,637 9 View

Binding of DNA to histone core octamer: SNP's and effects on binding ?

01 January 1970 7,413 3 View

VALUES and DESIGN: Can you suggest any literature that addresses engaging stakeholder or client values in the design process please?

Stakeholders are frequently engaged during the design process as a matter of course and their input frequently disregarded if incongruent with the designer's intentions, particularly in...

01 January 1970 7,953 16 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View