Dear Colleague(s)

I am not a histone biologist but have been asked to contemplate the feasibility - indeed sensibility  - of a histone binding assay:

In essence we are proposing to look at the binding of a 147bp nucleotide sequence that wraps around the histone octamer (H2A, H2B, H3 & H4) to constitute the nucleosome 'in vitro'. We were wondering if a SNP variant in a common allele would affect the affinity of the 147bp sequence for the histone octamer and whether that could be ascertained by immune precipitation; digestion of the protein and NGS of the recovered population to quantify a bard code tag; In other words chIP Seq.

To elaborate: If 1 SNP variant loosened binding would this affect the amount of DNA recovered as a bound fraction quantifiable by chIP Seq versus another SNP variant giving tighter binding; better recovery and hence more bar code tags in the final NGS analysis

If I am honest, given that nucleosomes must be able to bind a whole haploid genomes I would be surprised if a single base change would produce such a quantifiable affect (assayed by ChIP Seq)

Thanks for any advice and for dispelling any naivety I might be displaying in my proposed scheme of work !