Dear Colleague(s)
I am well versed with real time qPCR but have never actually worked with human heart tissue to determine relative expression of a GOI, viz cytochrome p450 2J2 variant.
In particular, I want to look at expression of 2J2 in total RNA derived from human heart tissue and intend to utilise Gapdh and B2 microglobulin as housekeepers; Both of which appear to have high expression in human heart.
As you know 3 housekeepers is considered a pre requisite and ideally > 3 should be evaluated by Norm finder to select the 3 with best and most consistent expression.
Can anyone therefore suggest at least another 2 house keepers in the context of total RNA from human heart and perhaps some tips/instructions on candidate selection using norm finder (which I only know about in principle)?
I should add I intend to assay by sybr green
Much obliged!
Dear Hall,
You can use beta-actin and alpha-tubulin as house keeping gene.
Kindly go through the link
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294216/
Hope for the best.
- Badges
- Science topic