Has anyone done cDNA transfection on human primary T cells successfully using electroporation?
I have a question about zinc chloride salt powder. When I put zinc chloride powder in an oven at a temperature of 50 degrees to dry it (because it may absorb moisture), after about an hour, the...
26 November 2020 3,751 3 View
I want to know what kind of microscope should I use to check the stability of dual emulsions (W/O/W)? upright or invert? Fluorescence or polarized or ordinary light? What field of vision should I...
12 November 2020 6,044 3 View
Hi everybody. I want to simulate a super molecule in a solvent like heptane in material studio. Solvent molecules are about 700 and are around the super molecule. How can I simulate these...
07 September 2018 7,643 4 View
when we prepare a hydroalcoholic extractof a plant, after evaporation in vacuum condition, we do not have a completely dried extract. What do you do for completely drying these extracts?
28 January 2018 8,328 2 View
I appreciate any information regarding your experience with dilute suspension (
14 January 2014 7,192 7 View
We want to protect the S of methimazole, does anyone have any suggestion?
22 November 2013 4,267 7 View
What is your idea about "negative intercept" in calibration curve in HPLC? Can you recommend a good book on this subject?
08 March 2013 485 4 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
03 March 2021 5,360 2 View
02 March 2021 3,060 3 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View