Has anyone done cDNA transfection on human primary T cells successfully using electroporation?
Dear Friends I hope you are well. "I'm interested in the concept of a 'Knowledge Graph of the Cold Chain.' If you have any files or information on this topic, could you please share it with me?...
05 March 2024 2,305 2 View
Can passive movement of a joint, for example plantar/dorsiflexion of ankle joint, elicit EMG activity in tibialis anterior and gastrocnemius muscles?
09 June 2023 8,007 4 View
Hi How to measure the concentration of free calcium in the cytoplasm of rat beta cells? Thanks
15 April 2023 3,122 7 View
Why chitosan glutaraldehyde hydrogel does not swell and absorb water after being placed in the refrigerator?
11 March 2023 1,607 3 View
Does anyone have experience with using Amaxa nuclefactor or Neon for electroporating CRISPR plasmids or any other vectors in primery cells?
28 February 2023 6,834 2 View
I was wondering how this system compares to the BioRad electric pulse system for delivery of plasmid components? what is the optimized buffer or what is your advice to imorove KD efficiency?
20 February 2023 3,767 0 View
Hello. Good Night Recently in class, students were asked a question that is important to me, and I heard some interesting answers. My question is, how can a woman perform many duties and...
05 December 2022 6,891 8 View
I have two small characteristic peaks in the NMR spectrum showing negative integration values. I would appreciate it if you could share your opinion.
28 November 2022 9,933 2 View
Sohrab Sepehri one of Persian poets says at the end of one of his tender and mystical poems called "water`s footsteps" : It is not our business to fathom the mystery of rose🌹 Perhaps our...
26 November 2022 6,979 90 View
I want to have an in vivo experiment in one lab and cryosectioning and SA-b galactosidase staining in another lab, for long-distance transportation of mice kidney samples, Does anybody have...
19 August 2021 3,603 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View