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The isolated T cells will be further activated and expanded in vitro for transduction (with chimeric antigen receptors). The ex vivo culturing will take about 2-3 weeks. I know that for isolating...
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Recently we faced an issue where we transduced primary mouse T cells with CAR constructs + a fluorescent tag. We tracked the expression of the tag on day 0, 2, and 4 post-transduction and the tag...
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