HPLC protein marker

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How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to preserve the different plant tissues for seconadry metabolite estimation and other bioassays ?

Greetings, I am a PhD Scholar, I want to analyze the metabolites present in different tissues of bitter melon plant (Momordica charantia). I have grown the plants and I have the plant sample ready...

28 August 2023 764 2 View

How to find exchange current density (i₀) for volcano plot from DFT for HER?

In many articles, the exchange current (i₀) value for the volcano plot can be found from the Norskov's assumption. When I used their values, I couldn't achieve their results. The exchange current...

08 August 2023 6,641 0 View

FOXP3 Gene Mutations: Statistical Analysis of Healthy Volunteers and Diseased Patients?

Dear experts, We have analyzed the FOXP3 gene mutation of 10 healthy volunteers and 13 diseased samples. Out of these, 3 healthy volunteers (30%) and 8 diseased patients (61.53%) were found to...

21 July 2023 1,365 2 View

How to synthesis high molecular weight polyacrylamide?

I want to synthesize high molecular-weight polyacrylamide. I tried synthesising PAM using acrylamide, 2-acrylamido-2-methylpropane sulfonic acid in alkaline median like ph 7-8. In this case, I...

13 June 2023 2,086 4 View

How to Do Statistical Analysis of a Dataset: Smokers Protected from Diseases?

Dear Expert, We have a single group of data consisting of 520 volunteers. All of them were smokers and protected from certain diseases. In such a scenario, we would appreciate your guidance on how...

04 June 2023 8,802 5 View

Volume of distribution of Ascorbic acid in human?

Dear experts, Our team is working on a new intervention for drug dependence and we require information on the volume of distribution (Vd) for ascorbic acid in humans for our project. Would you...

08 April 2023 1,259 0 View

Setting the oral dose based on pharmacokinetic parameters?

Dear Kinetic experts, I need one kind suggestion from you. One X compound with molecular weight of 200.1, is highly water soluble with oral bio-availability of 65-75%. In that case I want to...

02 February 2023 9,696 4 View

Co-creation of customer experience and consumer experience: is it possible?

The co-creation of customer experience and consumer experience. Is it possible to create a theory development on this concept? Please shower your suggestions on

17 February 2022 8,952 4 View

How to mask nitrobenzene odor?

I want to prepare odourless nitrobenzene can explain how to do it? what are the chemicals used to do that?

06 February 2022 9,421 0 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View