Lymhpoma cell culture issues, help?

Hi! I need help for some issues in growing mantle cell lymphoma cell lines (REC-1, MAVER-1, Granta-519, Mino, Jeko-1). I noticed that it is a little bit hard to maintain a stable cell culture:...

09 February 2021 7,402 11 View

Help to set up an in vitro Bioaccessibility assay?

Hi everyone! i am looking for protocols to evaluate the bioaccessibility of carotenoids in plant matrix. Ideally the best would be a quite easy protocol that could be used massively in the lab,...

02 February 2021 5,516 2 View

How seminal was the influence of Giuseppe Campos Venuti and Federico Oliva in Italy, France, Spain and other countries?

The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now? They reasoned and...

30 October 2020 5,450 4 View

Is Sytox (from Thermo Fisher Scientific) a good choice to dectect NETs in flow cytometry using whole blood?

I found several papers in the literature where Sytox (green, red, blue, or orange) is used to detect Neutrophil Extracellular Traps, but it's always used with purified neutrophils. Do you know if...

07 October 2020 5,865 2 View

Maximum concentration for HPLC???

Dear Collegues, I'm starting a new project and I need to know what is the maximum concentration for a sample that have to be processed by analytical HPLC. Thank you so much for the answers! Luca

01 October 2020 1,654 5 View

How to make ethyl cellulose water soluble?

Good evening, I'm looking for a way to make ethyl cellulose soluble in water. I read about a low ethoxylated formulation with a DS around 1-1,5 but I cannot find it in any shop. Is there anyone...

20 July 2020 8,592 1 View

Single cell RNA sequencing on immune cells infiltrated in tissues: what is the best way?

I am planning to investigate how immune cells infiltrated in certain tissues may change between different mouse models. If I don't want to focus on a specific immune cell type, is it possible to...

30 June 2020 1,834 3 View

Empatica E4 data processing tools and strategies?

Hello there! I am a Masters student working at EPFL with Empatica E4. For the moment, as both the device and the data from it are very new to me, I am testing the solidity of the signals. At first...

14 June 2020 6,185 2 View

Binary Classification - Machine Learning - When using which one?

Hey, I don't exactly understand how I make the decision to choose a SVM or a DNN or Linear Classifier for my binary classification problem. Are there any aspects to proove and then decide? Or is...

28 April 2020 4,728 4 View

Looking for (MSc course) syllabus in the field of social innovation / territorial development / urban studies . Can you suggest any material?

Social Innovation courses are often declined in a way that is often attached to social entrepreneurship or design methods (i.e. theory of change). Can you suggest any material on how to draw a...

06 February 2020 4,607 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View