hello Jorge
this can sometimes mean you are amplifying more than 1 specific product; invariably s second primer diner species: this will show as a second melt curve in your dissociation cycle at the end of your standard curve PCR cycle
Sounds odd but is a common feature of RT-PCR. Ideally, your efficiency should be 100% but often amplifications are somewhere between 95-105%. Deviations from the 100 in either direction reflect the highly dynamic processes underpinning the method, and primer dimers -as mentioned by Laurence- or minor unspecific amplification can cause such values. After all this method provides a good estimate of copy number, but the true value may be slightly different because of the method's routine experimental error.
Thanks very much to all of you (Laurence, Jonci nad Anja), your answer was very heplful for me. Best regards to everyone.
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