How would someone make glycerin suppositories so that they would be solid and clear?

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Does 3T3-L1 or H9C2 cell line can be used for atherosclerosis model study?

I am designing research related to atherosclerosis in vitro, but unfortunately the cell lines currently available in our laboratory are only 3T3-L1 and H9C2, Does anyone can suggest me what...

17 July 2024 6,373 1 View

¿ Algún experto en simulación de inversores ?

Simular una topología de inversor para aplicaciones de vehículos eléctricos en el software PSIM

20 June 2024 2,490 0 View

Explain how regional and international policies, instruments and mechanisms contribute to the country's elections process?

Give precise answers

16 June 2024 842 1 View

Explain why it is important to consider gender issues in election process?

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16 June 2024 1,688 1 View

Explain on transparency and accountability of election expensess and its impact to fairness and corruptive actions?

Give clearly answers

16 June 2024 8,668 0 View

How independence and accountability of the electoral management body play a critical role in ensuring free and fair elections?

Give precise answers

16 June 2024 9,948 0 View

What do old testament sanctuary system mean?

What is actually to discuss in old testament sanctuary system is, It origin, symbol and their meaning, significant rules, guidelines for operation and salvific implications.

11 June 2024 9,421 4 View

Como son los comportamientos de los indicadores clave de la DEMOCRACIA en cada uno de sus paises?

La variable sociedad civil es definida operativamente como el conjunto de organizaciones sociales que procuran vigilar el comportamiento de la democracia en el amito político. No se incluyen otros...

27 May 2024 4,091 3 View

What are the green procurement strategies?

what are the strategies green procurement? how can the be measured in relation to organizational performance

15 May 2024 6,548 3 View

Does epiphytic orchids establish relation with fungi when germinate?

Terrestrial orchids establish a relationship with fungi when germiate. Duw to their small size, they lack reserve substances and need a fungus to feed them, a relationship that can last the entire...

12 May 2024 1,559 2 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Concentration of gelatin as brain model ?

I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.

19 July 2024 3,095 0 View

What adhesive should I use to secure samples that float to the bottom of the well plate?

I'm currently working with gelatin sponges which have a tendency to float, however, ideally I would like them to remain at the bottom of the well plate as intend to vulture cells on them. Is there...

03 June 2024 7,728 2 View

Problem while making hydrogel from chitosan and gelatin ?

A uniform mixture of chitosan and gelatin is made by dissolving in acetic acid, after that the mixture is transferred to a plastic petri plate kept at -20 C for 24h and then lyophilized. But i am...

29 May 2024 7,367 4 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View

Alginate Gelatin Chitosan hydrogel??

How can prepare Alginate Gelatin Chitosan hydrogel?

05 May 2024 6,229 2 View

Why protein bands are unclear or invisible on SDS PAGE gel after staining?

Hi everyone! I have carried out SDS PAGE analysis many times. In the experiment, protein sample was hydrolyzed gelatin. After staining, only bands of protein marker (standard) were shown, whereas...

24 April 2024 6,444 1 View

How to dissociate zebrafish embryonic stem cells?

Hi all, I'm trying to isolate zebrafish embryonic stem cells after 3 hpf of zebrafish embryo's. I have a couple of questions. - I already tried Pronase to dechorionate, but it is very difficult....

15 April 2024 9,059 0 View

How can we decide the spinning condition when spinning core-shell electrospun fiber?

Dear all, I'm trying to fabricate core-shell electrospun fiber using 5wt%PCL in HFIP as a core solution and 6.5wt%Gelatin in HFIP as a shell solution. Feed rate of core is 0.4 mL/hr (20G) and that...

12 April 2024 7,046 0 View

Why is my GELMA opaque?

I have been trying to synthesize high methacrylation GELMA using the following protocol : Precise Tuning of Facile One-Pot Gelatin Methacryloyl (GelMA... However, I find that my GELMA is very...

24 March 2024 1,070 2 View