How will I get rid of Dnase I from trizol extracted total RNA and its Dnase I treatment for removing gDNA contamination ?

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Hua Xiao

You can perform heat inactivation and/or phenol/chloroform extractions. On the heat inactivation without RNA degradation, please check the following paper.

Biotechniques. 2000 Aug;29(2):252-4, 256.

Sk Riyazat Khadim

Thank you @ Hua Xiao

Mohammed Al-Nussairawi

You have two options

1- Run it on column then centerfedge it with the wash buffer ( this can apply for only Trizol Plus RNA Purification Kit , ThermoFisher Scientific )

2- or after completing the RNA Isolation

Good luck