How will I get rid of Dnase I from trizol extracted total RNA and its Dnase I treatment for removing gDNA contamination ?

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Do I need to normalise the RNA for qPCR?

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Total DNA-PK immunofluorescence on M059J cell?

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deleted the question.?

----deleted the question.

22 December 2019 4,752 2 View

What exactly we can infer from Charge Density Difference plot of a Hybrid system?

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Problem with the WB-F344 cel line. anyone had any experience ?

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How do I increase the yield of my PCR product?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

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What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

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How optimize the Size Exclusion Chromatography?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to address this issue in the analysis of Real-Time PCR results?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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How to perform statistical analysis of coefficients extracted from transform techniques?

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Hua Xiao

You can perform heat inactivation and/or phenol/chloroform extractions. On the heat inactivation without RNA degradation, please check the following paper.

Biotechniques. 2000 Aug;29(2):252-4, 256.

Mohammed Al-Nussairawi

You have two options

1- Run it on column then centerfedge it with the wash buffer ( this can apply for only Trizol Plus RNA Purification Kit , ThermoFisher Scientific )

2- or after completing the RNA Isolation

Good luck