Mohammed Al-Nussairawi

3 Questions 21 Answers 0 Followers

Questions related from Mohammed Al-Nussairawi

BL21(DE3) pLysE Chemically Competent Cells, Sigma Aldrich?

Do I have to add chloramphenicol to the LB ?? when using this particular expression cells ? I am going to express bacterial proteins , and i have inserted them individually into the vector...

23 July 2019 8,935 8 View

What is the best expression vector?

Hi, What is the best expression vector for expressing a protein coded gene into E.coli BL 21 (DE3) plyse chemical cells ? I know that pET vectors are the best choice but which one is good ? for...

10 June 2019 8,146 1 View

Dear biotechnologists, i am going to do a double digestion for a plasmid that contains the insert inside (GOI), by EcoRI and HindIII?

In other words, I am going to run a single digestion and double by EcoRI and HindIII, as well as to the control ( undigested plasmid ) i have the kit from ThermoFisher which contains EcoRI...

06 June 2019 4,917 2 View