Sk Riyazat Khadim

5 Questions 9 Answers 0 Followers

Questions related from Sk Riyazat Khadim

How will I get rid of Dnase I from trizol extracted total RNA and its Dnase I treatment for removing gDNA contamination ?

I need to do qRT PCR of severl genes in microalgal samples and presence of either gDNA or Dnase I would be perturbing to PCR.

20 September 2018 6,081 3 View

Can anyone detail me about how to quantify TAGs using TLC ?

I am searching for a TLC based protocol for quantifying the TAG content in solvent(chloroform) extracted lipids, how can I quantify the relative TAG amount in total lipids

31 January 2017 6,447 2 View

Which database can I get the sequence of dgat1 gene or protein of Chlamydomonas reinhardtii ?

I want to find out conserved domains of diacyglycerol acyltransferase in several algal species. But I am having difficulty to get the dgat sequences of C renhardtii. I will appreciate any help..

08 January 2017 5,462 2 View

Can anybody tell me how to spike the known amount of C17:0 ME with standard of FAME mixture in a GC-FID based quantification of derivatized lipids

I am working on total lipid quantification of microalgal system via in situ FAME derivatization but having difficulties in generating the calibration equations of the standards.

03 June 2016 9,560 5 View

How one can check the extracellularly secreted free fatty acids by organism that grows in liquid medium?

Given fatty acids are hydrophobic in nature.

13 May 2016 6,207 3 View