We want to induce fatty liver in rats and after successful induction of steatosis (Fatty liver) treat them and want to see the effectiveness of our treatment in Induced steatosis rats.
By putting the rats to High Fat High cholesterol diet (HFHCD) till 4 weeks you can see clear fatty liver induction and continued to 12 weeks fatty liver can be changed to NASH. Further, to see steatosis in liver, Oil red O (ORO) labeling is the best technique.