We are extracting DNA from aged blood samples using Qiagen DNA extraction Kit. Unfortunately, the DNA concentration that we've got is 5-6 microgram/ microliters at 260/280. Do you have any recommendation please?
Hi,
just for clarifying of your question. 5 micrograms per 1 microliter of for whole sample volume?
Dont you think 5 nanograms per 1 microliter?
Dear Marcela Krutova,
Yes, the DNA concentration we have got is between 5-6 ng/microliter.
In last elution step, you could eleute your DNA in low volume of elution buffer/ h20. This would help to further increase the DNA concentration.
try to increase the sample volume if possible and as Gopal Tiwari said to use less elution buffer. or you can use different preparation of isolations in low volume elution buffer and finally pulled up the DNA for better results
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