I need extinction coefficient of anti-HER-2 antibody. where can I find it?
Stjepan Krešimir Kračun
Anyone can suggest me a good reference book on cancer biology that covers drug delivery and discovery as well as basic concenpts of cancer biology?
25 June 2024 9,245 1 View
Hello, I have started working with Western blot recently and have difficulties getting the beta-actin band. I recently tried the steps for getting a beta-actin band from the intestinal sample of...
17 June 2024 3,750 4 View
I have loaded mesoporous silica nanoparticles with a drug and attached them to antibodies but after conjugation with antibodies, the nanoparticles loaded with the drug lose their functionality to...
12 May 2024 4,691 1 View
What is the most effective kit or method for extracting RNA from a very small number of cells?
07 February 2024 7,611 3 View
I need to treat healthy primary human mammary epithelial cells. When I thaw the cells, I obtain a very small number of cells that appear unhealthy. I have used Media 171 and MEGS (mammary...
07 February 2024 7,934 3 View
I need to measure the concentration of a drug in the mesoporous silica NPs. Is there any method to measure the content directly? I know we can use indirect method to measure it in the supernatant...
21 November 2023 4,404 0 View
I'm working on a routing and scheduling problem in the home care services context. I consider a break as a dummy patient, so routing and scheduling are also implemented for the break node (with...
24 August 2023 2,232 1 View
I am trying to coat gold nanostars with mesoporous silica shell but many AuNSs are coated with silica instead of one individual nanostar. Anyone has any idea why and what I can do to have one...
03 February 2023 6,210 3 View
Do you have a suggestion for choosing effective movement exercises in the field of working with children with learning disorder.
28 July 2022 2,992 2 View
Dears I want to interfere the autophagy pathway in osteoclasts using 3-MA and rapamycin. In the literature I found some controversial information about the concentrations. I would ask if...
29 June 2022 7,879 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View