How can we choose the right method for Gaussian calculations?

I'm not working on biomolecules, I work on Organic, Organometallic, and sometimes inorganic structures. Do you know a simple way to apply the right calculation method? Is there any article or...

10 December 2020 3,396 5 View

How to dissolve sodium hyaluronate, 95% in water?

Hi, I am trying to dissolve Sodium hyaluronate, 95% in water with a concentration of 2.7% (w/v). However, it gets gel instead. So far, I have tried elevated temperature water bath (around 40...

30 August 2020 8,428 3 View

I need a source about the different extraction methods and their effect on extraction of constituents in medicinal plants?

For example which method can extract polar and nonpolar constituents from medicinal plants easily?

07 May 2019 8,242 14 View

I have ordered some peptides and I need to add 5(6)-FAM to them. Anybody has any idea how i can do this?

These peptides are not on resin anymore and i do have Wang resin to maybe conjugate them back on the resin before attaching the dye but i am not quite sure how to do so! Is there a better way to...

11 March 2019 547 9 View

Sequence without ATGG?

24 August 2016 5,615 3 View

Why my breast cancer cell lines started to grow slowly and detach?

Does anyone has advices or explanation for the following? I have three cells line MCF-7, T47D and MDA231, when I received these cells in 25 cm flasks from friend's laboratory, they were adherent...

09 May 2014 7,125 11 View

DNA extraction of helico form biopsy

I faced with problem about extracting DNA of H.pylori directly form biopsy samples I am not sure about my method I am using CTAB-Chlrophorm method but i didnt get any result yet could you help...

16 July 2010 8,782 1 View

Is there anybody ,who have synthesized silica nanoparticles ?

for synthesizing silver-silica nanoparticles by sol-gel method , i have probem with it .

01 January 1970 3,708 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View