Agrobacterium-mediated transformation
Particle bombardment
I am doing qPCR but my HPRT1 Ct is not coming at the same point. I see a 2 cycle spread in Ct. I have checked my RNA quality A260/280, 230/280 values, did ethidium bromide gel. I used superscript...
04 July 2024 5,605 3 View
I am working with leishmania threonine synthase (LdTS) enzyme of aspartic acid pathway which is ~75kDa protein. I purified the protein and confirmed by western blot too. The condition used for...
25 March 2024 7,978 3 View
Hey there, currently I am working on threonine synthase enzyme of Leishmania. I have successfully cloned it into E.coli DH5alpha strain. I have also transformed it into E.coli BL21(DE3) strain. I...
02 February 2024 1,962 4 View
02 February 2024 6,431 3 View
Hi, I extracted total cellular RNA from HEK293T cells by using a commercial kit. I left samples untreated or treated with 2ul RNaseA/T1 mixture (invitrogen) in the presence of PBS or protein...
11 January 2024 2,362 4 View
What is a Systematic Literature Review (SLR) and mention its scientific procedure.
12 December 2023 4,877 5 View
I want to find the research paper on the grafting of goji berries as rootstock.
09 December 2023 7,211 1 View
Biofilm Formation
10 November 2023 484 3 View
Not been able to find any article on this. Help me to find out.
09 November 2023 1,540 1 View
Hi XRD and TEM experts It doesn't make sense to me but I still wanted to see if it's possible to have XRD crystallite size bigger than TEM particle diameter. I'm talking about high BET oxide...
31 October 2023 1,767 4 View
we are analyzing activity of an endolysin which has N-terminal domain involved in antibacterial activity. The recombinant protein is not showing activity, and we are wondering that whether His-tag...
06 December 2023 8,821 6 View
We are in the process of determining the title and making one-step growth curves for lytic phages, but we are at a point where dilutions up to 10ˆ-54 result in complete lysis.... no counting of...
17 April 2023 741 4 View
Hello, I work with recombinant proteins (endolysins) derived from lytic bacteriophages. This week I am doing some tests with my recombinant protein and I noticed that it has lost its lytic...
12 December 2021 7,931 3 View
Hi everybody, I am planning to test effect of pH on endolysin (phage enzyme) activity, so I have to prepare solutions with different pHs. To delete effect of buffer components on enzymatic...
12 September 2018 613 10 View
In order to combat bacteria associated with bovine mastitis, I plan to find some new lytic phages and new endolysin genes. Is it feasible and reasonable to induce some phages from clinical staph...
10 November 2017 5,425 3 View
What are the common primer's (F/R) used to detect bacteriophage endolysins (lysine) and tail holin?
11 November 2015 4,064 1 View
The journal article entitled "Potential of sequential treatment with minocycline and S. aureus specific phage lysin in eradication of MRSA biofilms: an in vitro study" by Chopra et al. (2015)...
21 June 2015 6,065 6 View
I am working on Bacteriophage as biocontrol agent. If I would like to know the endolysin gene, I would like to go for whole genome sequencing. But I do not know whether it is possible to go for...
18 February 2015 8,028 3 View
I find out following gene encoded proteins during genome analysis of newly isolated phage from sewage water :"putative endolysin , putative head protein , putative portal protein , putative...
01 January 1970 124 1 View
