How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

How to reconstruct a Lamb wave by a series of pulses superposed?

Lamb wave is useful for solid structure health monitoring, and it consists many modes. Unlike ultrasonic bulk wave, when given a pulse excitation (for example, 5-cycle sinusoidal pulse), only...

08 December 2020 5,440 8 View

How to ultrasonic HOPG blocks in organic solvent to graphene dispersion?

Hello everyone,I was using the method from Nair(DOI: 10.1038/NPHYS2183) to ultrasonic HOPG in an organic solvent(NMP).after 8h sonication,unlike usual graphite which change into graphene...

16 November 2020 7,839 3 View

Band size NFkB of app. 150kDa??

I made a western blot with the p65 NFkB antibody and the phospho p65 NFkB antibody from cell signalling. I get a band for both the phospho and non-phospho antibody at around 150kDa, how can this...

16 November 2020 1,948 3 View

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30 October 2020 961 3 View

What is the difference between endometriosis and ovarian cancer in terms of signaling pathway?

Hello Recently, I am curious to know about mechanisms of endometriosis and signaling pathways. I have a question about the difference between signaling pathways in benign tumors and malignant...

26 October 2020 3,891 6 View

Where can I get LN-308 cell line?

Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...

20 October 2020 3,004 3 View

How can i increase the power of the ultrasonic pizeoelcetric sensor to atomize a viscous liquid?

I use a piezoelectric sensor, a humidifier electronic circuit and a 32-volt power supply to generate high-frequency ultrasound waves. I use these waves to atomize the liquid. the problem is that...

16 October 2020 5,097 8 View

How to wash the LiF left by Ti3AlC2 etching?

I added 1 g Ti3AlC2 to 1g LiF and 20mL 9 M HCl and stirred it at 450 rpm at room temperature for 48 h.After deionized water washing and ultrasonic treatment, the product was obtained, but its XRD...

08 October 2020 7,612 4 View

What are the differences between an ultrasonic homogenizer and a vortex mixer? What are their ads and cons?

I want to suspense and mix my micro/nano-particles inside a solution. What are the differences between an ultrasonic homogenizer and a vortex mixture?

05 October 2020 5,606 3 View