There was some information on the internet. They say after 200 c it starts to decompose, and release different byproducts some of which even can be toxic. It was also mentioned that we can autoclave at 121c for 15 min. However during the autoclave I see the color change which can be the result of decomposition of glucose. It would be very nice if you have any contribution about this.
I usually sterilize 200 mM glucose in pure water 30' at 120 C and I add glucose to the medium after cooling. I hope that this will work also for you.
Normally 20-30min at liquid cycle (121C) works fine for 2M glucose in our hands. There can be a slight change in color, which may become more obvious if the bottle is left for too long after the pressure has come down (temp. remains near 100C); this does not seem to cause any obvious problem.
In case if you are very concerned or have reasons to believe that some oxidation is causing problems, you can always sterilize your stock glucose by passing through a 0.2 micron filter.
As you mentioned there was some color change. This color change increases depending on the concentration of glucose, Which means I have some percent decomposition. Since the color also may cause defects in our research, it maybe better to use the filtration instead. Thank you for your informative response.
Thank you all. Yeah I also understood that first I should autoclave the buffer then, filter glucose under hood and add to the solution.
By autoclaving change in color is obvious and stem from decomposing of glucose. However, the best approach to make glucose sterile is filtration by 0.2 micrometer, autoclaving can be used in many of cases.
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