When we are evaluating the remineralization in the interface layer between the sealant and enamel using scanning electron microscopy (SEM)could we assess in the same time the mineral content in a quantitative way of the interface enamel?
Dear Laith Alsabek , you could use the EDS detector if available in your SEM. It will give you qualitative and quantitative information about the elements present in your transversal section, so you could assess how the level of remineralization is. Bear in mind that this analysis provides information about a quite shallow volume of your sample, it is quite superficial and may be mineralization could be different at different points (sections) of your sample. For a better and easier way to see the possible remineralization you can use an EDS mapping, which will show you where each element is more or less concentrated in your sample.
Hope it helps.
Thank Manuel Gómez for your recommendation, it's really helpful! I guess the EDS mapping gives more insight about the infiltration depth of minerals, doesn't it?
EDS is informative method for elements mapping with advantage in one time measurements SEM images + elements content (using of special detectors, like INKA).
https://www.thermofisher.com/blog/microscopy/edx-analysis-with-sem-how-does-it-work/
Another independent method named TOF-SIMS can be useful.
https://www.thermofisher.com/us/en/home/materials-science/sims.html?gclid=CjwKCAjw7diEBhB-EiwAskVi17E1GPd7T1nQXrxg1upnv-gfaQ1VZ8nST0UVkWag-cPAWxfMaTrHfBoCxKwQAvD_BwE
You are right mapping EDS is good for infiltration of resin. However line analysis will give you qualitative analysis for the hybrid layer. Be aware that calcium And phosphorus peaks may overlap the gold peak which may be used for coating your specimen.
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