I would like to see beta cell apoptosis in human pancreatic dissociated cells. I thought I would make a costaining: TUNEL to detect apoptosis and Insulin to mark beta cell. Someone has ever done? Do you have any protocol?
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Goodwin G. Jinesh
Its better to do in serial sections. one for TUNEL and other for insulin. If not, go for TUNEL-FITC first, and then insulin-Cy3
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Cecilia Verga Falzacappa
Tunel is ok but it is mandatory that you use two different fluorescence channels, so the red one for beta cell markers. You can counterstain for either insulin or glut 2, it will mark beta cells too..
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Oscar Rafael Escate Chávez
Hello Nicola
You can try with Anexin V, where you cam detec the early apoptosis by polarization change in cell-membrane.
Regards
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