Hi! I'm looking for a way to verify the PCR amplification before capillary electrophoresis during a MLPA protocol. Many thanks!
260/280 nanodrop would say if dna was amplified compared to blank
Is there a repository with case studies of chemical processes modeled in state space?
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This is a process that is nowadays present in most of the energy production methods, what are the most promising solutions to work with something else?
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is it more efficient to use a battery with an electric engine, to use a hydrogen cell to conver to electricity on an electric engine or to use a hydrogen combustion engine?
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i am needing the correct way to write general order kinetic equation in origin, to do nonlinear fit
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Apparently, the existing adaptive models were developed for offices and schools.
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Free idea by Ingrid L. M. Souza Hello everyone, I would like to share freely my idea for the production of a vaccine. I would produce the vaccine with the peptide PRRARS|V present in the spike 2...
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
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A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
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I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
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I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
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Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
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How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
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