How to use interproscan5 for predicting domain based protein-protein interactions?

24 November 2015 0 6K Report

Hello

I'm working on protein-protein interactions (PPIs). First I retrieved the whole proteome of pathogen and host, then I upload the pathogen's proteome to the psortb server and got the results and I removed the cytoplasmic proteins and remains the positive candidate proteins (involve in PPIs). After this I again upload the whole proteome to the effector database to retrieve the secreted proteins, then I will find PPIs by two methods; domain based and interolog method. Can anyone help me in PPIs by domain based and interolog method?

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