Hi,
I have been facing problem with HUVEC attachment. I culture the cells on 1% gelatin coated cell culture flask. After 60-70% confluence, cells layer starts detach from the surface. Even fixing with 4% paraformaldehyde did not work. After every washing with PBS cells get washed out. Can anybody suggest how to fix the problem? I would appreciate if anyone suggest any other coating or culture condition. Thank you in advance.
-Ripon