03 September 2021 0 7K Report

Hi,

I have been facing problem with HUVEC attachment. I culture the cells on 1% gelatin coated cell culture flask. After 60-70% confluence, cells layer starts detach from the surface. Even fixing with 4% paraformaldehyde did not work. After every washing with PBS cells get washed out. Can anybody suggest how to fix the problem? I would appreciate if anyone suggest any other coating or culture condition. Thank you in advance.

-Ripon

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