We first isolate these specific T cells from your sample. Then, we give them a little "stimulation cocktail" – usually special antibodies that bind to their surface receptors (CD3 and CD28). This is like sending them a signal that it's time to get active and start growing. We also add some growth factors, like IL-2, to keep them happy and multiplying. This activation process, which usually takes about a day or two, makes the T cells more open to letting the AAV-GFP virus in and start making that green fluorescent protein we want to see with the flow cytometer.

Moreover, using a high viral titer, transduction enhancers, and selecting the appropriate AAV serotype are key to success. I encourage you to study these highlights I've made for you to support your successful results.

Great suggestions, thanks.