Sameer Tiwari

3 Questions 3 Answers 0 Followers

Questions related from Sameer Tiwari

How to transduce effectively with AAV particles in CD4 T cells?

I want transduce AAV-GFP in h-CD4 T cells to see the increased GFP by flow cytometry. Looking for the best protocol to increased infectivity of AAV in CD4 T cells.

05 May 2025 4,897 2 View

Which is the best protocol for isolation of AAV particles from adherent cells?

I want to isolate AAV particles from tranfected adherent cells. Would appreciate a best working protocol to isolate AAV particles using the kit based method or any minmium time consuming method.

28 March 2025 3,318 2 View

Could anybody share protocol for screening monocrotophos degrading actinomycetes?

Monocrotophos is insecticide used for agricultural purpose. One of my student working on actinomycetes which degrade them from toxic to non-toxic form.

01 July 2019 6,640 1 View