I had got contradictory results concerning the LC3 protein between western blot and Immunofluorescence. Any explanations?lanation?

Using western blot, LC3-II ( rerpresenting the autophagosomes or the autolysosomes) decreased in treated cells compared to control while using IF, LC3 puncta ( rerpresenting the autophagosomes or...

17 September 2020 6,453 3 View

Is it possible to combine art and science in one research?

Between an Art professor and a Biology professor for example

29 December 2019 3,138 4 View

Are There international recognized publishing journals in the field of painting?

I tried to publish in the field of Painting or fine arts but most Journals refuse because of that exact specialization

05 August 2019 2,870 3 View

Is POMS-2 available for free?

Is POMS -2 test pdf available for free? where?

24 March 2019 5,575 1 View

How can we remove excess of unadherent bacteria in invasion or adhesion assay ?

infection of host cells

10 February 2019 1,150 4 View

How to score the CCTST Form A?

Hello, Do any one used before the California Critical Thinking Skills Test- Form A? I am using it in my thesis; however i didn't find any info regarding scoring the test after giving it to...

30 August 2018 1,663 2 View

Is there a problem in the solubility of Methomyl 97% (Carbamates pesticide) in acetic acid or methanol, it gave a non-symmetric UV-Vis peak?

I used a Moleculary Imprinting Polymers (MIPs) technique on the Methomyl as a template and we need to create its calibration curve in different solvents. In aqueous media, it gave a sharp UV peak...

11 February 2018 6,238 2 View

What is the fastest way to learn using LaTex for scientific writing?

What platform to use? the editor name? the different editing formats and templates? is there a video course or lectures notes that is quick study to be able to write a full scientific paper in the...

08 February 2018 2,777 6 View

What is the fastest way to learn about Mendeley?

is there a document or video that is quick study to use Mendeley reference management software? Many thanks

06 February 2018 3,887 17 View

What is the fastest way to learn about neural networks?

What is the fastest way to learn about neural networks? there types? how to use them? full solved examples? real life applications? Many thanks

05 February 2018 8,107 14 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View