No. Tris-HCl is a buffer used to maintain a slightly basic pH. The reason it is Tris-HCl, is that Tris (tris-hydroxyaminomethane) is a base. HCl is added to lower the pH to the desired value.

HCl is a strong acid. If you used that without the Tris,. the pH would be way too low.

No, Tris is the buffer. HCl is used only to adjust the pH.

No, you cannot directly replace Tris-HCl with HCl in a TAE (Tris-acetate-EDTA) electrophoresis running buffer. Tris-HCl and HCl are different compounds with different properties and functions.

Tris-HCl (Tris hydrochloride) is a buffering agent commonly used in biological and biochemical experiments. It helps maintain a stable pH during electrophoresis. Tris-HCl acts as both a source of Tris, a weak base, and the chloride ion, which helps maintain ionic strength.

HCl (hydrochloric acid) is a strong acid and does not provide the buffering capacity necessary for electrophoresis. It would significantly lower the pH of the buffer, which could interfere with the separation and migration of nucleic acids during gel electrophoresis. Moreover, HCl does not provide the Tris component required for maintaining the desired pH range.

If you need to prepare a TAE running buffer and do not have Tris-HCl available, it would be best to obtain Tris-HCl or a TAE buffer kit from a scientific supplier or laboratory resource. Properly formulated buffers ensure reliable and consistent results during gel electrophoresis.

Hala Almshawit we may have misunderstood. Did you mean to replace the acetate with HCl? Instead of Tris-acetate use Tris-HCl?

Well, you could, but it would likely somewhat change the running conditions. Notably, there is also TBE, where the acetate is replaced with boric acid and while it works in principle similarly, it has different usage, because it behaves slightly different. The same would happen with Tris-HCl-EDTA buffer.

I'd recommend to use tris base instead and to add the acid (HCl or HOAc) yourself. More flexibility and usually saves tons of money.