how to tell if protein induction works?

14 October 2014 0 3K Report

I am not sure if my induction have worked. Is it possible to do a RNA miniprep. IF RNA successful extracted- means induction has worked.

Badges
Science method

More Shanice Chiang's questions See All

Why do you only consider one electrode for the equivalent circuit for fitting data of an EIS measurement?

I‘m currently trying to find a fit for my data but I‘m struggling with finding the correct equivalent circuit. I‘m working with a two-electrode-system and my electrolyte is my analyte. I can’t use...

26 September 2023 3,096 3 View

Dose MOAH include toluene and xylene?

As I know, MOAH is defined as Mineral oil aromatic hydrocarbons contain at least one aromatic ring in according to JRC 2019 (JRC115694). However, SGS told me that even if my ink sample contains...

09 February 2023 4,251 0 View

Are toluene and xylene MOAH?

As I know, MOAH is defined as Mineral oil aromatic hydrocarbons contain at least one aromatic ring in according to JRC 2019 (JRC115694). However, SGS told me that even if my ink sample contains...

25 January 2023 3,608 2 View

Why the peaks of emission spectra had not shifted as excitation wavelengths change indicated good monodispersion of FCP in size and fluorescence?

The paper shows "the corresponding emission spectra of blue and green FCP(fluorescent copolymer) at various excitation wavelengths showed that the peaks of emission spectra had nearly not shifted...

17 October 2021 1,133 2 View

Is elecrtopolishing necessary for AA6061 alloy?

DEAR ALL: Now I encounter a problem, every time I polish AA6061 alloy, there are lots of black dots on the surface. I have almost read a lot of articles that recommend them try to use...

08 April 2021 3,586 9 View

Does isocyanate react with TiO2 pigment in polyester?

Does the surface of TiO2 contain OH group that can react with isocyanate? This TiO2 pigment is dispersed in the polyester dissolved in organic solvent. I want to know if TiO2 interrupts the...

26 November 2020 7,552 5 View

Does anyone has manual for Burleigh Inchworm 8200 controller?

We have an old one lying around, and would like to bring it back to life.

24 November 2020 2,367 0 View

How to remove the resist residue after development of E-beam lithography?

Hi there. I've been working on fabrication of 100nm-width aluminum junctions by E-beam lithography and E-beam deposition. My questions is that there is always some dark shadows in the...

23 October 2019 8,737 6 View

Maximum number of cells for adoptive transfer of lymphocytes R/O?

Hello, I am wondering the maximum number of B cells one could adoptively transfer via the retro-orbital sinus into immunodeficient mice? Thanks, Kristy

17 February 2019 8,175 1 View

Modeling interface boundary condition in ANSYS Fluent?

Hi all, I am currently modeling a cylinder subjected to a gust of wind. While I figured out how to model a close top cylinder, I could not figure out how can I make the wind blow through the top...

28 December 2018 9,393 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View