i want to study root length, diameter, of wheat plant as affected by K nutrition. So please kindly provide me procedure for sample collection from field condition not in pot culture experiment.
Dig a hole beyond the root depth and gradually wash/brush away the soil to expose thr roots. This is not easily done even in sandy soils
You must provide care while uprooting the wheat plant from the soil. Suggested method is makes a dig around root peripheral area, filled with water and wash away the adhered soil particles, then transferred to lab rinse in water again. Then go for scanning analysis of harvesting root.. Scanning mainly possible in pot grown plant roots (because volume is less and damage to the root is meager)
Block trench with cube sampling can be applied using sharp metal sheets http://www.agriculturejournals.cz/publicFiles/21435.pdf, but you may add in situ hydraulic conductance. In brief you cut a section with known length and diameter and apply a positive water pressure by a tank with a Pitot tube. then you take the time for a given volume flux.
Thank you Dr.Gobinath. For how much depth i have to dig a hole?
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