I am working on probiotics and for my safety assessment part of the research i need to tag probiotics with antibiotics and for that I need suggestions or protocols if you have any. Thanks
All probiotic bacteria is gram positive , non catalase production, Resist the acidity and enzymes of the stomach and resists bile salts in the intestines. You can depend on these tests.
Antibiotic resistance expression and transferability of antibiotic resistance determinants from fed probiotic strains to commensal microbiota in vivo are important components of the safety assessment of bacteria used. Genetic stability of the probiotic overtime, deleterious metabolic activities, and the potential for pathogencity or toxigenicity must be assessed depending on the characteristic of the genus and species of the microcrobe being used. Immunological effects must be considered, especially in certain venerable populations including infants with underdeveloped immune functions. For more details consult https://www.ncbi.nlm.nih.gov