Hello everyone,
I am carrying out an experiment that nanoparticle synthesis by desolvation method. For the bonding i target, firstly i need to keep pH of the reaction at 8-10. I use bovine serum albumin. In literature, they add NaOH on BSA before desolvation. However, if i add NaOH before desolvation, the pH return to the neutral condition after addition of the solvent as expected so, i have to add NaOH after adding desolvating agent. If experiment is conducted in pure water (i mean if i dissolve BSA in pure water and i add desolvating agent on it) turbidity is completely loss after i add NaOH. In PBS, turbidity is not completely lost, but a small loss of turbidity is still observed and some of proteins aggregate.
Why this situations are observed? I couldn’t understand the effect of NaOH on protein and is there any advice to keep nanoparticle synthesis at basic conditions?
Dear Eda Argıtekin, NaOH role is to introduce irreversible structural/conformational changes on the protein structures. Please check the following document. My Regards
https://doi.org/10.1016/j.molliq.2020.113065
Dear Eda Argıtekin;
Please check the following document.
With my best regards
Hello Eda,
thank you for sharing this very interesting technical problem with the RG community. In addition to the useful references suggested by Abdelkader BOUAZIZ and Doufnoune RACHIDA Rachida please also have a look at the interesting article cited below. It contains a detailed protocol for the preparation and purification of BSA nanoparticles by the desolvation method:
BSA nanoparticles as controlled release carriers for isophethalaldoxime palladacycle complex; synthesis, characterization, in vitro evaluation, cytotoxicity and release kinetics analysis:
Article BSA nanoparticles as controlled release carriers for isophet...
Unfortunately this paper has not yet been posted as public full text on RG. However, three of the author have RG profiles. Thus it should be easy to request the full text directly from one of the authors via RG (provided that it is of interest to you).
Good luck with your work and best wishes!
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